Prions, proteinase K and infectivity
نویسندگان
چکیده
منابع مشابه
Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid
Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using proteinase K, however it is now apparent that the majority of disease-related PrP and indeed prio...
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BACKGROUND The transmissible agent of Creutzfeldt-Jakob disease (CJD) is not readily destroyed by conventional sterilization and transmissions by surgical instruments have been reported. Decontamination studies have been carried out thus far on solutions or suspensions of the agent and may not reflect the behavior of surface-bound infectivity. MATERIALS AND METHODS As a model for contaminated...
متن کاملSome physico-chemical parameters that influence proteinase K resistance and the infectivity of PrPSc after high pressure treatment.
Crude brain homogenates of terminally diseased hamsters infected with the 263 K strain of scrapie (PrP Sc) were heated and/or pressurized at 800 MPa at 60 degrees C for different times (a few seconds or 5, 30, 120 min) in phosphate-buffered saline (PBS) of different pH and concentration. Prion proteins were analyzed on immunoblots for their proteinase K (PK) resistance, and in hamster bioassays...
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The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K sta...
متن کاملComparative Studies on the Interaction of Proteinase-K with Fe2O3, Fe3O4 and SiO2 Nanoparticles
The interaction of Fe2O3, Fe3O4 and SiO2 nanoparticles with proteinase K activity was investigated using UV–vis spectroscopy. Proteinase K EC (3.4.21.14) is a member of serine protease family, which is produced from fungus Tritirachum album Limber.The effects of nanoparticles on proteinase...
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ژورنال
عنوان ژورنال: Prion
سال: 2012
ISSN: 1933-6896,1933-690X
DOI: 10.4161/pri.22309